Orderly nucleic acid aggregates by electrostatic self-assembly in single cells for miRNA detection and visualizing.
نویسندگان
چکیده
Orderly nucleic acid aggregates (ONAAs) self-assembled on mesoporous silica nanoparticles (MSNs) with positively charged aminopropyl groups (PC) were firstly developed. Interestingly, a novel electrostatic DNA self-assembly could realize hybridization chain reaction (HCR) on the surface of PCMSNs in single cells. Significantly, a non-destructive amplification strategy based on ONAAs-PCMSNs was successfully developed for miRNA detection and in situ imaging by the prominent and agminated fluorescence-bright spots in living cells.
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عنوان ژورنال:
- The Analyst
دوره 141 10 شماره
صفحات -
تاریخ انتشار 2016